Fig. 2
From: Review of the technology used for structural characterization of the GMO genome using NGS data
Comparison of NGS techniques for evaluating the genome structure of Eukaryotes and Prokaryotes. A NGS analysis techniques for evaluating the genome characteristics of eukaryotic LMOs: to assess the genome characteristics of eukaryotic LMOs, a shotgun library was created from the whole genome of the event and sequenced. Mapping these sequences to the plasmid backbone revealed integration sites where T-DNA was exclusively attached. B NGS analysis techniques for evaluating the genome characteristics of prokaryotic LMOs: • De novo assembly (left): During de novo assembly, contigs are formed, allowing for the confirmation of sequence depth at each locus. • Genome structure evaluation (right): This method distinguishes between scenarios in which T-DNA is integrated solely into the plasmid and cases in which it integrates into both the plasmid and the bacterial chromosome. Confirmation of the genome structure involves analyzing the PCR results and the composition of the genome