Table 2 Summary of different methods of cell preparation and isolation strategy
From: Navigating single-cell RNA-sequencing: protocols, tools, databases, and applications
Method | Principle | Advantages | Limitations | Applications |
|---|---|---|---|---|
FACS [38] | Fluorescence-based sorting using specific cell markers | Highly selective, precise isolation | Expensive, cellular stress | Targeted cell populations |
Microfluidics (droplet-based) [39] | Encapsulation of cells in droplets with barcoded beads | High-throughput, efficient, automated | High cost, transcript loss | Large-scale profiling, general use |
Split-pooling [40] | Combinatorial barcoding without physical isolation | Cost-effective, highly scalable | Complex data handling, barcode collisions | Large-scale studies, multiplexed samples |
snRNA-seq [41] | Isolation of nuclei instead of intact cells | Minimal dissociation stress, suitable for frozen tissues | Lower RNA yield, excludes cytoplasmic transcripts | Difficult to dissociate tissues, archival samples |